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When you set the BaseDataBoundControl.DataSource property, the BaseDataBoundControl.ValidateDataSource(object) method is called. In addition, if the data-bound control has already been initialized, the BaseDataBoundControl.OnDataPropertyChanged method is called to set the BaseDataBoundControl.RequiresDataBinding property to true.
This property cannot be set by themes or style sheet themes. For more information, see System.Web.UI.ThemeableAttribute and ASP.NET Themes Overview.